Phototransduction gain at the G-protein, transducin, and effector protein, phosphodiesterase-6, stages in retinal rods
نویسندگان
چکیده
منابع مشابه
Interaction between cGMP-phosphodiesterase and Transducin a-Subunit in Retinal Rods
Cross-linking of the different subunits of the retinal cGMP-phosphodiesterase (PDE) with its activator GaGTPrS (a subunit of the retinal G-protein transducin with GTPrS (guanosine 5’-0-(3-thiotriphosphate) bound) has been investigated using purified proteins, with a N-hydroxysuccinimide homobifunctional crosslinker, bis(sulfosuccinimidy1)suberate (BS3) and its cleavable analog 3,3’-dithiobis(...
متن کاملPhototransduction mechanism in retinal rods and cones. The Friedenwald Lecture.
V ision begins in the rod and cone receptors of the retina, where light is absorbed and a neural signal is generated. This signal, in the form of an electrical hyperpolarization of the cell membrane, influences second-order visual neurons by modulating the rate of neurotransmitter (glutamate) release from the synaptic terminal of the photoreceptor. This release is high in the dark and is reduce...
متن کاملG Protein Deactivation Mechanisms in Vertebrate Phototransduction
H.-Y. Tu Institute of Molecular Medicine, National Tsing-Hua University, No. 101, Section 2, Kuang-Fu Road, 30013 Hsinchu, Taiwan, Republic of China e-mail: [email protected] Abstract Heterotrimeric G proteins are widely used in nature to facilitate cellular responses to extracellular stimuli. In humans, these G proteins mediate vision and other senses, modulate neurotransmission, and are...
متن کاملReceptor and bg Binding Sites in the a Subunit of the Retinal G Protein Transducin
Transmembrane receptors for hormones, neurotransmitters, light, and odorants mediate their cellular effects by activating heterotrimeric guanine nucleotide–binding proteins (G proteins). Crystal structures have revealed contact surfaces between G protein subunits, but not the surfaces ormolecularmechanism throughwhichGabg responds to activation by transmembrane receptors. Such a surface was ide...
متن کاملGain and kinetics of activation in the G-protein cascade of phototransduction.
The guanine nucleotide binding protein (G protein) cascade underlying phototransduction is one of the best understood of all signaling pathways. The diffusional interactions of the proteins underlying the cascade have been analyzed, both at a macroscopic level and also in terms of the stochastic nature of the molecular contacts. In response to a single activated rhodopsin (R*) formed as a resul...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2019
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1904017116